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All About Polyclonal And Monoclonal Antibodies

Both monoclonal and polyclonal antibodies can be used in a variety of different applications, including Western blot, ELISA, immunoprecipitation, immunofluorescence and immunohistochemistry. Each method has advantages and disadvantages to consider when selecting your antibody.

Polyclonal antibodies are usually produced in rabbits, goats, chickens or mice after activation of multiple B cell clones. To know more about polyclonal and monoclonal antibody, you can also browse Therefore, polyclonal immune serums contain a group of antibodies with different chemical and physical properties that recognize multiple epitopes of the immunized antigen.

Advantages of polyclonal antibodies:

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Since antibodies bind to different protein epitopes, proteins that are expressed in small amounts can be more easily detected because the signal is amplified.

Antibodies are more tolerant of minor changes in the nature of the antigen, such as polymerization or mild denaturation.

General considerations for polyclonal antibodies:

• Preferably, when the type of protein is unknown

• Preferred choice for detecting denatured proteins

• Variability from batch to batch between different animals

Detect proteins with high homology. To ensure specificity, the antigenic sequence should be checked for significant cross-reactivity

The immune response in animals immunized with the same antigen will vary individually. Therefore, the general recommendation for the production of polyclonal antibodies is immunization of at least two animals with the antigen.

Monoclonal antibodies are usually produced by single B cells from immunized BalbC mice. Fusion of B cells and myeloma cells creates a hybridoma cell line that produces antibodies that recognize a single epitope with low cross-reactivity.

Advantages of monoclonal antibodies:

• Usually low cross-reactivity

• Facilitate standardization in manufacturing processes, such as: B. Clinical diagnostic and treatment kits

General considerations for monoclonal antibodies:

• Can produce an unlimited number of specific antibodies

• High homogeneity

• Preferably for protein detection in tissue

• Preferably for analysis requiring quantitative assessment